NGS Services Spectrum

NB! If the service you need is not the list below or the required protocol is not among the established procedures, please nevertheless do contact us, together we will find a solution.

Assistance in experimental design

  • Project planning (what is feasible depending on the task, quality and amount of samples, resources available, etc.)
  • Sample processing procedure
  • Library preparation protocol
  • Optimal sequencing platform and sequencing mode
  • Analysis solutions
  • Costs & time estimation

Sample processing

  • DNA and RNA quantification (Qubit)
  • DNA and RNA QC (BioAnalyser, TapeStation, Fragment Analyser)
  • Sample preparation for single-cell applications
  • Tissue dissociation (e.g. using Miltenyi gentleMACS)
  • FACS (BD FACS Aria III, BD FACS Aria Fusion, Miltenyi MACSQuant Tyto)
  • Cell separation (dead cell and debris removal, targeted enrichment)
  • Cell and tissue storage and conservation/fixation
  • Rare cell handling (e.g. cellenOne, Miltenyi OctoMACS)
  • Nuclei isolation
  • Single-cell suspension handling and cell counting

Sequencing libraries preparation

Illumina NGS PacBio NGS Single cell NGS
  • TruSeq stranded mRNA (manual and automated)
  • TruSeq stranded total RNA (manual and automated)
  • TruSeq small RNA
  • TruSeq ChIP-Seq
  • NEBNext Ultra2 mRNA
  • NEBNext Ultra 2 total RNA
  • SMART-Seq (Takara)
  • Agilent SureSelect AllExon
  • Nextera
  • Amplicon library
  • ISO-Seq library
  • Whole genome library
  • 10X Genomics Chromium
  • Takara ICELL8 (NanoDispenser or cellenONE)
  • 1Cell-Bio inDrops
  • Fluidigm C1
  • FACS-seq (Biomek Fxp)
  • Mission Bio Tapestri (soon)
  • BD Rhapsody (soon)

Sequencing libraries QC

  • Concentration measurement (Qubit)
  • Size distribution (BioAnalyser or TapeStation)
  • qPCR (KAPA library quantification protocol)

Sequencing

Illumina NGS PacBio NGS
  • NovaSeq (available from the middle of February 2019)
  • 2x HiSeq 4000
  • 3x NextSeq 500
  • MiniSeq
  • Sequel

Data Analysis

  • Demultiplexing and QC
  • Raw data/fastq files transfer
  • Alignment
  • Functional analysis (project-specific)

Consulting & troubleshooting

  • Optimal DNA and RNA isolation procedures
  • Good laboratory practice for sequencing library preparation
  • Troubleshooting unsuccessful sequencing experiments
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